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. 2013 Sep;19(9):1208–1217. doi: 10.1261/rna.039446.113

FIGURE 4.

FIGURE 4.

Mutation in RPL1B suppresses CGA-mediated inhibition of downstream gene expression. (A) Yeast strains bearing (CGA)4-ura3 and (CGA)4-his3 genes exhibit a His−Ura− phenotype that is suppressed by expression of the exact base-pairing mutant tRNAArg(UCG). The indicated strains bearing a CEN plasmid with or without mutant tRNAArg(UCG) were grown overnight in SD-leu media, and serial dilutions were spotted on the indicated media and grown at 25°C or 33°C. (B) Deletion of the RPL1B gene in the (CGA)4-ura3 and (CGA)4-his3 parent results in a His+, Ura+ phenotype, which is complemented by plasmid-borne copies of either RPL1B or RPL1A. (C) Expression of (CGA)4-firefly luciferase is increased nearly threefold in the rpl1b-1 mutant. (D) The inhibitory effects of CGA codons at amino acid 318 are suppressed in the rpl1b-Δ mutant. The mean GFP/RFP from a Renilla-(Arg)4-GFP fusion was compared with (AGA)4 and (CGA)4 insertions at amino acid 318 in the wild-type and rpl1b-Δ mutant.