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. 2013 Sep;19(9):1226–1237. doi: 10.1261/rna.037077.112

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© 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 3. — Effect of magnesium ions on the resistance of pRNA 3WJ to urea denaturation, assayed by 15% PAGE gels with (A) 2 mM EDTA and (B) 5 mM MgCl₂. (Lane 1) Single RNA oligo b_3wj; (lane 2) sample annealed from two oligos a_3wj and b_3wj; (lane 3) 3WJ assembled from three oligos a_3wj, b_3wj, and c_3wj; and (lane 4) DNA ladder.