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. 2013 Sep;19(9):1226–1237. doi: 10.1261/rna.037077.112

FIGURE 4.

FIGURE 4.

Structure of a pRNA hexamer ring and docking of the hexameric pRNA model with the dodecameric connector ring of phi29. (A) A 3D model of hexameric pRNA based on the crystal structure of 3WJ with views from three mutually perpendicular angles. (B) AFM images of hexameric re-engineered pRNA rings show strong correlation in size and shape with the 3D computer models for the intact, full-length pRNA hexamers. (C) Histogram of RNA particles in AFM images with discernible stoichiometry. (D) Top view of the published 3D computer model of hexameric pRNA constructed based on biochemical data (PDB 1L4O) (Hoeprich and Guo 2002). (E) Model of a pRNA hexamer complexed with a dodecameric connector ring based on the 3WJ and connector (PDB 1H5W) (Guasch et al. 2002) crystal structures. (For an animation movie of the phi29 pRNA-connector model, see Supporting Video S1.) (F) A close-up view of proper anchoring of the connector N-terminal helices for optimal interactions with pRNA. (G) Three mutually perpendicular views of the hexameric pRNA-connector assembly. (H) A side-view of the 3D model of the hexameric pRNA-connector assembly based on biochemical data (PDB 1L4P) (Hoeprich and Guo 2002).