FIGURE 7.

The helicase domain plays a central role in the cytoplasmic retention of Dicer. (A) HeLa cells were transfected with the indicated constructs. Twenty-four hours post transfection, cells were treated with 20 nM LMB or solvent (ethanol) for 10 h and fixed, followed by IF using an α-myc antibody. IF against RIOK2 served as a positive control for the LMB treatment. Bar, 20 μm. (B) Quantification of the experiment shown in A. Cells displaying either cytoplasmic localization or nuclear accumulation of the constructs were counted. (N) Number of counted cells.