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. 2013 Sep;19(9):1266–1278. doi: 10.1261/rna.039123.113

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© 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 7. — Mutational analysis of EBS-IBS interactions in Pe.th.I2. (A) Wild type EBS1-IBS1 and EBS2-IBS2 regions are shown. (B) Splicing of Pe.th.I2 variants with sets of three consecutive point mutations of the EBS1-IBS1 and EBS2-IBS2 interactions. Mutated residues are highlighted in capitalized bold characters. Time points of the splicing reaction indicated for WT also apply for mutant splicing reactions. Precursors (E5-I-E3), linear introns (lin-I), and 5′-exon (E5) are labeled according to their predicted sizes.