Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics and/or genotypea | Source or reference |
|---|---|---|
| Strains | ||
| R. eutropha | ||
| HF371 (RH+, AH+) | hoxGΔ hoxHΔ; MBH− SH− RH+ AH+ | 51 |
| HF500 (AH+) | hoxGΔ hoxHΔ hoxCΔ; MBH− SH− RH− AH+ | 26 |
| HF579 | hypA1B1F1C1D1E1XΔ hypA2B2F2Δ hoxGΔ hoxHΔ; MBH− SH− RH− AH+ | 52 |
| HF864 (AH++) | hoxGΔ hoxHΔ hoxCΔ, PMBH-hofK; MBH− SH− RH− AH++ | This study |
| HF866 (AH−) | hoxGΔ hoxHΔ hoxCΔ hofGΔ; MBH− SH− RH− AH− | This study |
| HF868 (AH−, RH+) | hoxGΔ hoxHΔ hofGΔ; MBH− SH− AH− RH+ | This study |
| HF901 (AHStrep) | hoxGΔ hoxHΔ hoxCΔ, PMBH-StrephofK; MBH− SH− RH− AH++ | This study |
| E. coli | ||
| JM109 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/e14− (McrA−) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17(rK− mK+) relA1 supE44 recA1 | 19 |
| S17-1 | Tra+ recA pro thi hsdR chr::RP4-2 | 20 |
| Plasmids | ||
| pET24a | Kmr PT7 expression vector | Novagen |
| pLO1 | Kmr sacB, RP4 oriT, ColE1 ori | 21 |
| pCH1676 | PMBH with flanking sequences of 0.8 kb upstream of the AH operon and N-terminal part of hofK in pET24a | This study |
| pGE814 | 3.0-kb EcoRV-SmaI fragment from pCH1676 in pLO1 | This study |
| pGE815 | 3.3-kb ′hofK-hofG-PHG066 fragment in pLO1 | This study |
| pGE816 | Religated 9.5-kb fragment from SexAI-digested pGE815 | This study |
| pGE817 | 2.2-kb ′hofK-Strep-hofG′ fragment in pLO1 | This study |
a
Kmr, kanamycin resistance.