Fig 2.
Purification of L. plantarum Lp_0796 protein. Shown is SDS-PAGE analysis of the expression and purification of His6-Lp_0796 and analysis of soluble cell extracts of IPTG-induced E. coli BL21(DE3)(pURI3-TEV) (lane 1) or E. coli BL21(DE3)(pURI3-TEV-0796) (lane 2), flowthrough from the affinity resin (lane 3), or fractions eluted after His affinity resin (lanes 4 to 8). The arrow indicates the overproduced and purified protein. The 12.5% gel was stained with Coomassie blue. Molecular mass markers are on the left (SDS-PAGE standards; Bio-Rad).