Fig 3.

Enzymatic activity of L. plantarum Lp_0796 protein. The hydroxycinnamoyl esterase activity of purified Lp_0796 protein (column 2) was compared with control reactions in which the enzyme was omitted (column 1). Shown are HPLC chromatograms of Lp_0796 (100 μg) incubated in 1 mM methyl ferulate (A), methyl caffeate (B), methyl p-coumarate (C), and methyl sinapinate (MS) for 10 h at 30°C. The methyl ferulate (MF), methyl caffeate (MC), methyl p-coumarate (MpC), methyl sinapinate (MS), ferulic acid (FA), caffeic acid (CA), p-coumaric acid (pCA), and sinapic acid (SA) detected are indicated. The chromatograms were recorded at 280 nm.