Fig 5.

Some biochemical properties of Lp_0796 protein. (A) Relative activity of Lp_0796 versus temperature. (B) Relative activity versus pH. (C) Thermal stability of Lp_0796 after preincubation at 22°C (diamonds), 30°C (squares), 37°C (triangles), and 45°C (circles) in phosphate buffer (50 mM, pH 6.5). At the indicated times, aliquots were withdrawn and analyzed as described in Materials and Methods. The experiments were done in triplicate. The mean values and standard errors are shown. The observed maximum activity was defined as 100%. (D) Relative activities of Lp_0796 after incubation with 1 mM concentrations of different additives. The activity of the enzyme incubated in the absence of additives was defined as 100%.