Fig 1.

Alignment of two primers and the TaqMan MGB probe sequences against the internal transcribed spacer (ITS) and cerato-platanin (CP) gene for which the assay was designed. Primer sequences are indicated by the arrow boxes, and the probe sequences are shown in the shaded boxes. The Ceratocystis platani reference sequences were EU426554 for ITS and EF017218 for CP. The sequences of C. cacaofunesta (CBS15362), C. fimbriata (114723), C. populicola (115161), and C. variospora (114719) were obtained in this study.