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. 2013 Sep;79(17):5394–5404. doi: 10.1128/AEM.01484-13

Table 4.

Reproducibility of ITS and CP standard curves

Parameter Standard curve result fora:
ITS
 CP
Serial dilution (pg/tube) (n = 24) CT ± SD %CV CT ± SD %CV
    2 × 104 14.22 ± 0.05 0.39 18.12 ± 0.38 2.12
    4 × 103 15.29 ± 0.13 0.85 20.16 ± 0.67 3.33
    800 17.09 ± 0.07 0.39 22.72 ± 0.71 2.85
    160 19.45 ± 0.19 0.96 25.02 ± 0.71 2.85
    32 21.87 ± 0.25 1.15 27.31 ± 0.80 2.94
    6.4 24.60 ± 0.23 0.95 29.70 ± 0.89 3.00
    1.28 26.87 ± 0.28 1.05 32.25 ± 0.96 2.98
    0.25 29.50 ± 0.56 1.90 35.05 ± 1.08 3.09
Other parameters (n = 7) Mean ± SD %CV Mean ± SD %CV
    Slope 3.22 ± 0.01 0.39 3.30 ± 0.03 0.94
    y-intercept 27.01 ± 0.06 0.21 32.23 ± 0.62 1.91
    Value of fit (r2) 0.987 ± 0.005 0.55 0.993 ± 0.01 0.59
    Efficiency 1.03 ± 0.01 0.55 1.02 ± 0.00 0.30
a

Standard curves for eight 1:5 serial dilutions were constructed with Cp5 reference DNA. The DNA control was diluted in water to cover a linear range of five orders of magnitude (from 2 × 104 to 0.25 pg/tube per sample). Each curve was run in triplicate in seven independent assays. Reproducibility was assessed by computing the coefficient of variation (%CV) among the mean values in seven independent assays. The efficiency of the standard curves was calculated based on the slope from seven independent experiments.