Table 4.
Parameter | Standard curve result fora: |
|||
---|---|---|---|---|
ITS |
CP |
|||
Serial dilution (pg/tube) (n = 24) | CT ± SD | %CV | CT ± SD | %CV |
2 × 104 | 14.22 ± 0.05 | 0.39 | 18.12 ± 0.38 | 2.12 |
4 × 103 | 15.29 ± 0.13 | 0.85 | 20.16 ± 0.67 | 3.33 |
800 | 17.09 ± 0.07 | 0.39 | 22.72 ± 0.71 | 2.85 |
160 | 19.45 ± 0.19 | 0.96 | 25.02 ± 0.71 | 2.85 |
32 | 21.87 ± 0.25 | 1.15 | 27.31 ± 0.80 | 2.94 |
6.4 | 24.60 ± 0.23 | 0.95 | 29.70 ± 0.89 | 3.00 |
1.28 | 26.87 ± 0.28 | 1.05 | 32.25 ± 0.96 | 2.98 |
0.25 | 29.50 ± 0.56 | 1.90 | 35.05 ± 1.08 | 3.09 |
Other parameters (n = 7) | Mean ± SD | %CV | Mean ± SD | %CV |
Slope | 3.22 ± 0.01 | 0.39 | 3.30 ± 0.03 | 0.94 |
y-intercept | 27.01 ± 0.06 | 0.21 | 32.23 ± 0.62 | 1.91 |
Value of fit (r2) | 0.987 ± 0.005 | 0.55 | 0.993 ± 0.01 | 0.59 |
Efficiency | 1.03 ± 0.01 | 0.55 | 1.02 ± 0.00 | 0.30 |
Standard curves for eight 1:5 serial dilutions were constructed with Cp5 reference DNA. The DNA control was diluted in water to cover a linear range of five orders of magnitude (from 2 × 104 to 0.25 pg/tube per sample). Each curve was run in triplicate in seven independent assays. Reproducibility was assessed by computing the coefficient of variation (%CV) among the mean values in seven independent assays. The efficiency of the standard curves was calculated based on the slope from seven independent experiments.