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TELCA was applied to lysates of V. cholerae strains ectopically expressing an active DGC (VCA0956; filled squares), an active PDE (VC1086; empty squares), and an empty vector control (empty circles). [13C]c-di-GMP was added to each lysate, and the hydrolysis was tracked over time using LC-MS/MS. A nonlinear regression using a one-phase exponential decay model was applied to each lysate. Each point represents the mean of three replicates; error bars indicate standard errors.
