Fig 6.

(A) TELCA was applied to lysates of WT V. cholerae and three different mutants. [¹³C]GTP was added to each lysate, and the amount of [¹³C]c-di-GMP synthesis was quantified using LC-MS/MS. (B) TELCA was applied to lysates of WT V. cholerae and three different mutants. [¹³C]c-di-GMP was added to each lysate, and the amount of [¹³C]c-di-GMP hydrolysis was quantified using LC-MS/MS. The percent loss was calculated by comparing each lysate to the product of a no-protein control reaction. All cultures were grown in triplicate, and the error bars indicate the standard deviations from the mean. Brackets indicate statistical significance determined by using a one-tailed Student t test (P < 0.05).