Fig 6.

Anti-Ly6G treatment results in depletion of neutrophils in the blood and lungs of RSV-infected mice. BALB/cJ mice were treated with IgG2a control antibody or anti-Ly6G antibody. These mice were mock infected or infected with 1 × 10⁶ PFU RSV 2-20. Blood was taken at days 1 and 7 postinfection. Lungs were harvested on days 1 and 8 postinfection. Cells were isolated by use of a Ficoll gradient and stained for CD11b and Gr-1. (A) Representative flow plots of PBMCs from the indicated group on day 1 postinfection. (B) Representative flow plots of PBMCs from the indicated groups on day 7 postinfection. (C) Quantification of PBMCs on day 1. Results are representative of those from three replicate experiments. (D) Quantification of PBMCs on day 7. Results are representative of those from three replicate experiments. (E) Representative flow plots of lung cells from the indicated group on day 1 postinfection. (F) Representative flow plots of lung cells from the indicated group on day 8 postinfection. (G) Quantification of lung cells on day 1. Results are representative of those from three replicate experiments. (H) Quantification of lung cells on day 8. Results are representative of those from three replicate experiments. Bars, significant differences (P < 0.05, ANOVA).