Fig 7.

1A8 administration does not affect macrophage numbers in mock- or RSV-infected mice. BALB/cJ mice were treated with IgG2a isotype control antibody or anti-Ly6G antibody. These mice were mock infected (n = 3 or 5 per group) or infected with 1 × 10⁶ PFU of 2-20 (n = 3 or 5). Lungs were harvested at day 6 postinfection Cells were isolated by the use of a Ficoll gradient and stained for MHC-II and CD11b. Data were analyzed using FlowJo software. (A) Representative flow plots gated for MHC-II⁺ CD11b⁺ cells; (B) quantification of three replicates.