Fig 2.

Anti-CLDN genotype-independent inhibition of HCVpp infection of 293T cells engineered to express CLDN receptors. CLDN1-, CLDN6-, and CLDN9-293T cells were preincubated for 1 h at 37°C with (A and B) serial dilutions of or (C) a fixed dose (100 μg/ml) of a CLDN1-, CLDN6 (WU-9E1-G2)-, CLDN9 (YD-4E9-A2)-specific, or control MAb before infection with HCVpp (strains H77 [genotype 1a], HCV-J [1b], UKN3A1.28 [3a], and UKN4.21.16 [4]) or MLVpp for 4 h at 37°C. Pseudoparticle entry was assessed as described in the legend to Fig. 1. Means ± SD from three independent experiments performed in triplicate are shown. Statistical analysis relative to the control MAb was performed using the Student t test, *P < 0.05.