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Isotherms for binding of RESTV VP35 RBD molecules to 18-bp dsRNA with blunt ends, 18-bp dsRNA with a 3′ overhang, and 12-bp dsRNA with blunt ends. The top of each panel shows enthalpic heat released during titration, and the bottom panel shows integrated binding isotherms with the proposed fitted model of binding. Binding events A and B are labeled in the leftmost isotherm.
