. 2013 Sep;87(18):10385–10388. doi: 10.1128/JVI.01452-13

Table 1.

Thermodynamic parameters of binding determined by ITC for binding of RESTV VP35 to dsRNA molecules that vary in length and the presence of a 3′ overhang at each end^a

RESTV VP35 dsRNA	ΔG^Ab (kcal/mol)	n^A	K_D^A (μM)	ΔG^B (kcal/mol)	n^B	K_D^B (μM)	n^AB
18 bp	−8.9	0.9	0.3	−7.6	6.9	2.8	7–8
18 bp + 3′ overhang	Unmeasurable^c	0.2	N/A	−7.8	7.8	2.0	7–8
12 bp	−9.6	1.2	0.1	−7.5	3.6	3.0	4–5

All values represent averages of two independent measurements. Superscripts A and B refer to events A and B wherever applicable. n represents the number of molecules binding in that event.

The change in Gibbs free energy (ΔG) was determined as follows: ΔG_binding = RTlnK_D (29).

There are too few measured points to confidently determine the ΔG_binding or the K_D of the first binding event (stoichiometry of <0.2).