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Transcriptase and minus-strand synthesis in wild-type particles packaging wild-type plus strands of segments S and M along with modified segment L plus strands that would result in transcripts starting with sequence GU, GA, GC, or GG. Reactions were performed under normal conditions of 1 mM manganese and 5 mM magnesium ions or at a high (3 mM) manganese ion concentration with 1 mM magnesium ions.
