Fig 8.

qRT-PCR analysis of the expression of HEX1 and its orthologs in F. graminearum and Cryphonectria parasitica as affected by mycovirus infection. (A) Relative HEX1 mRNA expression in the WT-VF, WT-VI, Δhex1-VF, Δhex1-VI, HEX1 OE-VF, HEX1 OE-VI, Δhex1::HEX1-VF, and Δhex1::HEX1-VI strains of F. graminearum. Relative transcript levels were normalized using cDNA of elongation factor 1α and cyclophilin 1. (B) Relative mRNA expression of HEX1 homologs in C. parasitica. EP155, virus-free C. parasitica mating type Mat-2; UEP, EP155 infected with CHV1; EP155-FgV1, EP155 infected with FgV1. Error bars indicate standard deviations. Values with different letters are significantly different at a P value of <0.05 based on the Tukey test.