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Antigenicity of GLA cross-linked gp160. HEK293-A cells were transiently transfected with UG37 gp160ΔCT (clade A) or mock transfected as indicated. Antibody binding to unmodified (WT) or cross-linked (GLA) gp160 was analyzed by CELISA. Representative titration series of MAbs (epitopes are indicated in parentheses) show maintenance (HIVIG, VRC01, 2G12, and PG9) or selective decreases (17b and F105) of binding. Lines indicate results of the nonlinear fit where possible.
