Fig 4.

Immunogenicity of GLA cross-linked CN54 gp140. (A) CN54 gp140-specific total IgG endpoint titers of sera from New Zealand White rabbits (n = 8 per group) immunized with unmodified (WT) or GLA cross-linked (GLA) CN54 gp140 showed no significant differences at any time point (one-way ANOVA of log-transformed data with Bonferroni's posttest). Inject represents time points of immunizations. (B) Binding of week 24 sera to cell surface-expressed heterologous ZM53 Env was analyzed by fluorescence-activated cell sorting (FACS). **, P < 0.01 according to a t test of log-transformed data. (C) Binding of sera to a GLA cross-linked or unmodified irrelevant protein (HEL) was analyzed with week 24 sera, and results are expressed as an index of binding to GLA-treated/untreated HEL. Student's t test was performed for statistical analysis.