Fig 7.

Neutralization assays. (A) Neutralization of week 24 sera from animals immunized with unmodified (WT) or GLA cross-linked (GLA) gp140 was tested in the TZM-bl assay against tier 1 viruses (left) and in the more sensitive A3R5 assay against tier 2 viruses (right). (B) Magnitude-breadth curves are shown for pooled data from all animal sera from both assays. A one-way ANOVA of log-transformed data with Bonferroni's posttest (A) and a Mantel-Cox log-rank test (B) were used for statistical analysis. *, P < 0.05; **, P < 0.01; ***, P < 0.001.