Fig 8.

Characterization of neutralizing responses. (A) Neutralization of HIV-1 strain MW965 by week 24 sera in the presence of V3 peptide (V3), scrambled control (Scr), or vehicle alone (PBS) in TZM-bl cells. (B) Neutralization curves of MW965 by week 24 in the presence of V3 peptide plus either the CD4bs probe RSC3 or the corresponding negative control, ΔRSC3. Curves show averages of all animals from the two groups. (C) Loss of neutralization upon addition of inhibitor is shown as the ratio IC₅₀ (no V3 loop)/IC₅₀ (V3 loop) (left) or IC₅₀ (V3 + ΔRSC3)/IC₅₀ (V3 + RSC3) (right). A one-way ANOVA of log-transformed data with Bonferroni's posttest (A), a curve-comparison F test (B), and a Mann-Whitney test (C) were used for statistical analysis. *, P < 0.05; **, P < 0.01.