Fig 4.

Furin dependence of Y47/48A growth and E1 surface expression. WT or mutant SFV RNA was transfected into WT (A) or furin-deficient FD11 (B) CHO cells. Cells were cultured for the indicated times, and progeny virus production was quantitated by plaque assay on BHK cells. At 24 h posttransfection, cells were fixed with paraformaldehyde, and E1 surface expression was visualized by indirect immunofluorescence using a MAb to E1 (C). Fluorescence microscopy images were acquired with the same exposure times. Scale bar, 10 μm. Data are representative of the results of two to three independent experiments.