Fig 4.

17-DMAG decreases phosphorylation of EBV PK substrates. (A) AGS-Akata cells were treated with 17-DMAG or the DMSO control for 48 h. Whole-cell extracts were prepared 2 days later, and immunoblot analysis was performed to analyze the expression of BMRF1, BZLF1, Cdc2, and actin, as indicated. (B) AGS-Akata cells transfected with the vector control or BZLF1 (in the presence or absence of 17-DMAG) were examined by immunoblotting using antibodies to detect phosphorylated serine 22 of lamin A, total lamin A, EBV PK, transfected BZLF1, and tubulin. (C) HeLa cells were transfected with the vector control, a myc-tagged expression vector for EBV thymidine kinase (EBV TK) (150 ng), or the EBV TK expression vector (50 ng) and the EBV PK expression vector (50 ng) in the presence or absence of 17-DMAG. Immunoblot analyses were performed to examine EBV TK, EBV PK, and actin expression. (D) Mutu I Burkitt lymphoma cells were treated with and without TGF-β to induce lytic infection (in the presence or absence of 17-DMAG), and immunoblot analyses were performed to detect the expression of EBV PK, EBV TK, and actin.