17-DMAG decreases expression of multiple CHPKs. (A) HeLa cells were transfected with the empty vector control or vectors expressing UL97, HHV6 PK, HHV7 PK, KSHV PK, RCMV PK, MHV68 PK, or EBV PK (all tagged with HA). A 48-h treatment with 17-DMAG (0.17 μM) or the DMSO control began at 4 h posttransfection. Whole-cell extracts were prepared, and immunoblot analyses were performed to analyze the expression of PKs using antibodies against HA, Cdc2, or actin, as indicated. (B) HeLa cells were transfected with the SG5-UL97 (HA-tagged) vector in the presence or absence of 17-DMAG, and different amounts of the protein extracts were loaded onto a gel, as indicated, and examined by immunoblotting using antibodies directed against UL97 (HA antibody) and actin.