Fig 9.

17-DMAG decreases intracellular lytic EBV DNA replication. (Left) AGS-Akata cells were treated with 17-DMAG (0.17 μM), the DMSO control, and/or acyclovir (50 μg/ml) for 2 days, and the DNA was harvested. The DNA was cut with BamHI and hybridized to a probe spanning the EBV termini for Southern blotting. The forms of the EBV genome containing fused viral termini (a mixture of latent and lytically replicated genomes) as well as the cleaved linear forms of the genome (produced only by lytically replicated viral DNA) are indicated. (Right) Immunoblotting was performed to ascertain the protein levels of BMRF1 and BZLF1 in the AGS-Akata samples used in the left panel.