Fig 10.

Histone modification of BZLF1/BRLF1 promoters in mSP1 and mMEF2 mutant LCLs. LCLs latently infected with recombinant EBV-BAC were cross-linked and subjected to ChIP experiments as described in Materials and Methods using normal IgG and anti-histone H3, -H3K9Ac, -H3K4me3, -H3K9me2, -H3K27me3, -H3K9me3, and -H4K20me3 antibodies, followed by DNA extraction and real-time PCR to detect DNA fragments using the indicated primers. The Zp/Rp numbers indicate the sequence positions relative to the transcription start site. As controls, the TR region of EBV, the β-globin promoter (Globinp), and the GAPDH promoter (GAPDHp) were also quantified.