Fig 2.

Library screening identified factors involved in BZLF1 promoter activation. (A) HEK293T cells were transfected with 10 ng of the reporter plasmid pZp-luc, 1 ng of pCMV-RL, and 100 ng of expression plasmids for the indicated genes. (B) As in panel A, HEK293T cells were transfected with 10 ng of the reporter plasmid pZp-luc (wt) or its promoterless parental vector, pGL4.10 (empty); 1 ng of pCMV-RL; and 100 ng of expression plasmids for the indicated genes. Luciferase assays were carried out as described in Materials and Methods. Luciferase activity is shown as fold activation over that with the control vector. Each bar represents the mean and standard deviation (SD) of three independent transfections.