Fig 5.

Expression of viral proteins from recombinant viruses in HEK293 cells. The recombinant EBV-BAC DNAs shown in Fig. 5 were introduced into HEK293 cells, followed by hygromycin selection. The resultant cell clones were tested for viral protein expression. Two or three clones of each recombinant virus were transfected with the BZLF1 expression vector (pcDNABZLF1) or its empty control vector. After 24 h, cell proteins were harvested and immunoblotting was performed using anti-BZLF1, -BRLF1, -BALF2, -BALF5, -BMRF1, and -tubulin antibodies.