Fig 3.

Viral IL-10 upregulates expression of M2c-associated mRNA transcripts in human monocytes. CD14⁺ monocytes were treated with viral IL-10 (100 ng/ml) or with PBS (negative control) for 24 h. (A) Fold mRNA expression change of M2c-associated transcripts SLAM, versican, and SOCS3 in monocytes treated with viral IL-10 (relative to PBS-treated samples). (B) Fold mRNA expression change of M1-associated transcript CCR7 and M2a-associated transcript fibronectin in monocytes treated with viral IL-10 (relative to PBS-treated samples). The number of independent biological replicate experiments (n) is shown. Error bars indicate the standard errors of the means. Significant differences between samples compared to PBS treatment were determined using a one-tailed, paired Student t test: *, P < 0.05; ***, P < 0.001.