Fig 5.

Blocking the human IL-10 receptor on CD14⁺ monocytes prevents surface CD163 upregulation by viral IL-10. CD14⁺ monocytes were pretreated with anti-hIL-10R antibody for 2 h prior to incubation for 24 h with conditioned supernatants from HFF cultures productively infected with HCMV strain AD169 (A) or recombinant viral IL-10 (100 ng/ml) (B). Surface CD163 expression with anti-hIL-10R antibody (area under the dashed line) was compared to the surface CD163 expression from the identical treatments but with anti-hIL-10R antibody omitted (A, mock) and treatment with PBS with anti-hIL-10R antibody omitted (B, PBS; negative control). Error bars indicate the standard errors of the means. Significant differences between samples compared to PBS treatment were determined using a one-tailed, paired Student t test (*, P < 0.05).