Fig 2.

IBDV VP3 colocalizes with endocytic markers. Transfected or untransfected HeLa cells were infected and processed for indirect immunofluorescence analysis with anti-VP3 (1:500) and the corresponding Alexa 488-conjugated (green signal in untransfected cells) or Cy3-conjugated (red signal in EGFP-Rab5 [e to h]-, EGFP-Rab7 [i to l]-, and EGFP–LAMP-1 [u to x]-transfected cells) secondary antibodies. In untransfected HeLa cells, double immune staining was performed with the corresponding endosomal marker as indicated. The nuclei are stained with Hoechst stain (blue). The insets show the colocalization of VP3 with the cellular marker in the perinuclear region. Images are representative of three independent experiments. Scale bars represent 10 μm.