Fig 6.

Cholesterol-tagged peptide interacts with F glycoprotein. Monolayers of HeLa cells coexpressing HN and F were allowed to bind to receptor-bearing RBCs at 4°C. Upon transfer to 37°C, medium containing AU, CAU, scrambled peptide, or no peptide was added. Zanamivir was added to block HN-receptor interaction at the indicated time points, and RBCs that were reversibly bound by HN-receptor interaction (A, P ≤ 0.05), irreversibly bound (B, P ≤ 0.05), or fused (C) were quantified. The ordinate values are means ± standard deviations of triplicate samples.