Skip to main content
. 2013 Aug;87(16):9233–9249. doi: 10.1128/JVI.01145-13

Fig 1.

Fig 1

Comparison of VLP Env and soluble gp120 by BN-PAGE. The Env components of various VLPs and soluble gp120 were resolved by BN-PAGE-Western blotting and detected with anti-gp120 and anti-gp41 MAb cocktails. Ferritin was used as a size marker (GE Healthcare). Diagrams depict native trimer, UNC gp160 monomer (mostly gp160ER), soluble gp120, and gp41 stumps. Complex glycans are indicated by blue “tree” structures and oligomannose glycans by green “tree” structures. The SOS disulfide bond is depicted by red bars that link gp120 and gp41. Monomeric gp160ER, bearing largely only untrimmed high-mannose glycans, is a major form of Env on all VLPs. The faint doublet of oligomers on UNC SOS-VLPs (lane 3) is likely to include trimers of mature gp160 and gp160ER. Recombinant gp120 is largely monomeric, with a fraction of dimer.