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. 2013 Aug;87(16):9323–9332. doi: 10.1128/JVI.01581-13

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Fig 3 — Characterization of the recombinant viruses constructed for this study. (A) HEL cells mock-infected or infected with wild-type HSV-1F, YK421 (ΔICP22), YK422 (ΔICP22-repair), or YK423 (MEF-ICP22) at an MOI of 5 for 18 h were analyzed by immunoblotting with antibody to ICP22, Flag epitope, ICP0, ICP27, or α-tubulin. HEL (B) and Vero (C) cells were infected with HSV-1F, YK421 (ΔICP22), YK422 (ΔICP22-repair), or YK423 (MEF-ICP22) at an MOI of 5. Total virus from the cell culture supernatants and infected cells was harvested at the indicated times and assayed on Vero cells.