Fig 6.

Effect of p53 reintroduction into HCT116 p53^−/− cells on viral replication. (A) HCT116 p53^+/+-empty, HCT116 p53^−/−-empty, and HCT116 p53^−/− p53⁺ cells were analyzed by immunoblotting with antibody to p53 or α-tubulin. (B) HCT116 p53^+/+-empty, HCT116 p53^−/−-empty, and HCT116 p53^−/− p53⁺ cells were infected with wild-type HSV-1F, YK421 (ΔICP22), or YK422 (ΔICP22-repair) at an MOI of 0.01. Total virus from cell culture supernatants and infected cells was harvested at 48 h postinfection and assayed on Vero cells. P values were calculated by a two-tailed Student t test.