Fig 8.

Effect of the p53-null mutation on expression of ICP27 mRNA in infected cells. HCT116 p53^+/+ and HCT116 p53^−/− cells infected with wild-type HSV-1F at an MOI of 5 were harvested at the indicated times, and the amounts of ICP27 mRNA were analyzed by quantitative RT-PCR. Each bar is the mean ± standard error of data from three independent experiments. The mean value for HCT116 p53^−/− cells was calculated relative to that for HCT116 p53^+/+ cells, which was normalized to 1. P values were calculated by a two-tailed Student t test.