Fig 9.

Effect of p53 by itself or p53 in combination with VP16 on the ICP27 promoter. HCT116 p53^−/− cells were transfected with pRL-CMV and pICP27-luc (ICP27 promoter) (A) or pSRα-luc (SRα promoter) (B) in combination with pcDNA5/FRT (empty), pcDNA-VP16 (VP16), and/or pcDNA-p53 (p53). At 24 h after transfection, luciferase activity was assayed. Relative promoter activity was calculated as firefly luciferase activity/Renilla luciferase activity. The results were the means ± standard errors from the results of triplicate experiments. Data are representative of three independent experiments. P values were calculated by a two-tailed Student t test.