Fig 4.

Activation of miR-203 by immediate and delayed signaling pathways. (A) A549 cells were infected with Sendai virus (5 PFU/cell), and the abundance of miR-203 was measured at indicated times after Sendai virus infection by TaqMan miRNA RT-qPCR. (B) A549 cells were stimulated with IFN-α (1,000 units/ml) or infected with Sendai virus (SeV) (5 PFU/cell) for 4, 10, and 24 h in the presence (+) or absence (−) of cycloheximide (CHX 100 ng/ml; 1 h pretreatment and continuous thereafter). Abundances of miR-203 and miR-449b were determined by TaqMan microRNA assays at the indicated time points. Statistical significance was determined by a two-tailed t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001. M, mock; hpt, hours posttransfection.