Fig 3.

pM79 localizes to replication compartments during infection. MEF10.1 cells were mock infected or infected with SMgfp or SM79flag at an MOI of 2. At 24 hpi, cells were fixed with methanol (which quenches GFP fluorescence) and stained with either rabbit polyclonal (A) or mouse monoclonal (B) anti-FLAG antibody for detection of the tagged M79 protein (green). In the experiment shown in panel A, cells were costained with antibody to pM44 to mark replication compartments (red). Images in the last row of panel A are magnified views of an infected nucleus where the M79 protein (pM79) localized. Cells were counterstained with TO-PRO3 to visualize the nuclei (blue). Scale bar, 20 μm. Shown is a representative result from four reproducible, independent experiments.