Fig 2.

Vaccine-induced IgM helps to prevent the spread of pathogenic RABV into the CNS. Groups of sIgM^−/− or wild-type mice were immunized with rRABV-ΔM and then challenged with CVS-N2c as described in the legend to Fig. 1. Five days postchallenge, total RNA was isolated from the muscle (A), the spinal cord (B), and the brain (C) and analyzed by qRT-PCR for the presence of CVS-N2c-specific viral mRNA. Data represent a combination of two independent experiments (total n = 8 mice/group). One-way analysis of variance (ANOVA) was used to evaluate statistical significance. Where significant differences were observed, Tukey's multiple comparison test was used to identify significant differences between individual groups (*, P < 0.05).