Table 2.
Chicken no., expt no. | Virus titer (log10 EID50/g of tissue) |
||||
---|---|---|---|---|---|
Lung | Spleen | Heart | Brain | Muscle | |
1, 1 | 7.1 | 5.1 | 6.1 | 5.1 | 6.1 |
2, 2 | 6.8 | 5.9 | 6.5 | 5.8 | 5.5 |
1, 2 | 7.1 | 5.7 | 7.1 | 6.5 | 4.7 |
2, 2 | 6.3 | 5.1 | 5.3 | 5.5 | 4.1 |
Tissues collected at 2 days postchallenge were homogenized to a 10% (wt/vol) final concentration. Tenfold dilutions of the 10% homogenates (100 μl) were inoculated into 10-day-old embryonated chicken eggs (ECE), and virus titers were calculated. The threshold of detection was 101.9 EID50/g of tissue.