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EV1 infection requires cholesterol. (A) Caco-2 cells were pretreated with MβCD or MβCD plus cholesterol before infection with EV1, EV7, or CVB3. (B) Cells were pretreated with MβCD before exposure to EV7 (early) or at 2 h postinfection (late). (C) Cells were pretreated with filipin before infection with EV1, EV7, or CVB3. Means ± SD of three independent experiments performed in triplicate are shown. **, P < 0.01.
