Fig 6.

EV1 infection does not depend on caveolin- or clathrin-mediated endocytosis. (A) Caco-2 cells transfected with siRNA targeting caveolin-1 (siCav-1) were infected with EV1 or CVB3. (B) Caco-2 cells transfected with plasmids expressing GFP-tagged wild-type caveolin 1 (WT) or a dominant negative form (Y14F) were infected with EV1 or CVB3; results for Y14F cells were normalized to those obtained for control cells expressing the WT. (C) Caco-2 cells transfected with siRNA targeting clathrin heavy chain (siCHC) were infected with EV1 or CVB3 or incubated with labeled transferrin (TFN). (D) Caco-2 cells transfected with plasmids expressing GFP-tagged wild type (WT) or dominant negative (DN) Eps15 were infected with EV1 or VSV; results for DN cells were normalized to those obtained for control cells expressing the WT. (E) Caco-2 cells were pretreated with 10 to 20 μg/ml of chlorpromazine before infection with EV1 (MOI of 2), EV7 (MOI of 2), CVB3 (MOI of 3), or VSV (MOI of 2). In all graphs, means ± SD of at least three independent experiments performed in triplicate are shown. **, P < 0.01.