Fig 9.

Virus entry depends on factors associated with macropinocytosis. (A) Cells were treated with inhibitors and permitted to bind EV1 (MOI of 200) for 45 min in the cold and then washed and incubated for 30 min at 37°C to permit virus entry; cells were then fixed, permeabilized, and stained with anti-EV1 (green) and anti-EEA1 antibodies (red). (B) Cells treated with inhibitors were infected as described in panel A; at 30 min p.i., cells were fixed, stained with anti-EV1 without permeabilization (Unperm; red), and then refixed, permeabilized, and restained with anti-EV1 (Perm; green). Magnified images of boxed areas are shown in the lower panels.