Fig 2.
Expression of HCMV gO isoforms by replication-defective adenovirus (Ad) vectors. U373 cells were transduced with Ad vectors expressing gO isoforms (HA tagged) derived from HCMV TR (TRgO), Merlin (MEgO), TB40 (TBgO), AD169 (ADgO), and Towne (TNgO). Cells were labeled for 5 min with [35S]methionine-cysteine, and then the label was chased for 0 or 180 min. Proteins were immunoprecipitated with anti-HA antibodies, left untreated (−), or treated with endo H (H) or PNGase F (F), and separated by SDS-PAGE (8%) under reducing conditions. Arrows and arrowheads mark untreated and deglycosylated forms of gO, respectively. Mass markers (kDa) are shown on the left.