Fig 3.

Western blot detection of gO isoforms by antipeptide rabbit sera. Primary human fibroblasts were transduced with Ad vectors expressing the indicated gO isoforms (HA tagged). Cell extracts were analyzed by Western blotting using anti-HA antibodies (A) or antibodies raised against peptides derived from gO of HCMV TR (B), Merlin (C), TB40 (D), or AD169 (E). For detection with antipeptide antibodies (B to E), long and short chemiluminescent detections (top and bottom, respectively) were performed in parallel, allowing comparison of signal intensities. Mass markers (kDa) are shown on the left.