Fig 4.

Effects of distinct gO isoforms on the intracellular trafficking of a common isoform of gH/gL. U373 cells were transduced with Ad vectors expressing gH/gL (derived from TR) alone or together (+) with Ad vectors expressing gO isoforms derived from TR (TRgO), Merlin (MEgO), TB40 (TBgO), AD169 (ADgO), and Towne (TNgO). Cells were pulse-labeled with [³⁵S]methionine-cysteine, and then the label was chased for 0 or 180 min. Proteins were immunoprecipitated with anti-gH MAb 14-4b, left untreated (-), or treated with endo H (H) or PNGase F (F), and separated by SDS-PAGE under reducing conditions. Arrows mark untreated or endo H-resistant forms of gH and gL. Arrowheads mark deglycosylated forms of gH and gL. Mass markers (kDa) are shown on the left.